Tissue Culture | Piruby

Home/Tissue Culture | Piruby
Tissue Culture | Piruby 2018-03-28T06:53:03+00:00
Modern agriculture is on the brink of a new era one that represents a quantum leap into the 21st century. Although there will always be a need for conventional agricultural methods, there’s no doubt that the green revolution of a quarter century ago is rapidly giving way to today’s gene revolution. The cornerstone of this new biotechnology is the propagation method known as tissue culture

Tissue culture

is a relatively new laboratory science which encompasses a variety of procedures and techniques. Simply stated, tissue culture is the method by which plant and animal cells and tissues are grown in a sterile media.

Scientists are broadening their perspectives to utilize

tissue culture

in nearly all phases of agriculture with tremendous possibilities. For example, in the food industry tissue culture provides means by which protein-rich sweeteners are being developed for baked goods, jams, ice cream, and candies. This will raise the nutritional value of these foods. Aided by tissue culture plant pathologists have created experimental bacteria that inhibit frost formation on plant leaves. Soon strawberries and other tender crops will be able to sustain temporary periods of low temperatures without damaging the crops. Scientists have made spectacular advancements through tissue culture researchers, and now are working on vegetables like tomatoes that withstand herbicide sprays and thrive in salty soils. Corn hybrids capable of producing their own nitrogen fertilizer and Super grains that tolerate extended drought.


tissue culture

is more widely used in commercial horticulture than in any other area of agriculture. It was the orchid industry, that more than 25 years ago first used tissue culture on a large scale. Orchids are notoriously slow to reproduce, and their seeds produce variable plants to compound the problem.

Professionals are finding that

tissue culture

produces genetically identical plants. These plants are economical, requires less labor space and convenient to maintain than following traditional methods, for example, one grower uses tissue culture to produce1000 daylilies each week on just 30 square feet of shelf space. Another grower using conventional propagation methods would need 1/2 acre to produce the same number of daylilies.

Tissue culture techniques also enable growers to produce vast numbers of plants. When mother stock is in short supply as another example Dutch iris normally produce about five daughter bulbs per year under optimum conditions. With tissue culture, you could produce several hundred Dutch irises from a single bulb within three months. The potential for rapid mass production makes tissue culture particularly appealing in commercial horticulture. Tissue cultured plants have guaranteed health status, it’s possible to transport plants into countries formerly inaccessible due to strict quarantine regulations. This means we can look forward to the rapid worldwide introduction of new and improved plants in the not-too-distant future.

On a research level, horticulturists are using tissue culture to create new plants from totally unrelated families. They’re also able to alter the number of chromosomes in plants. They can breed plants with improved form vigor and production techniques such as gene transfer. Gene transfer was once regarded as science fiction has become reality by fusing into one cell the contents of two genetically different cells.

Another new technique is called embryo rescue plants that fail to produce viable seed is also part of tissue culture. By this method before the fertilized ovary or immature seed degrades an embryo rescue is a valuable tool in the production of new lilies

With mutagenesis, scientists can induce mutations in an effort to develop plants resistant to disease, cold toxins, and herbicides. Tissue culture also enables long and short-term germplasm storage. With this method, the genetic material of old and rare plants can be preserved from extinction.

Rounding out the newer tissue culture techniques in horticulture is encapsulation this involves the formation of embryos from single identical cells. In the laboratory, the artificial embryos are coated with a gel that may contain fertilizers herbicides, pesticides, growth regulators, and fungicides. The resulting seeds produce identical plants giving uniform results at low cost.

Potentially any living cell in the tissue of any plant can grow into a normal plant through tissue culture because such small pieces of plant tissue are used in tissue culture, the procedure is often referred to as a type of micropropagation it’s equally well known as in-vitro propagation.

The tissue obtained from the mother plant is called eggs plant. The resulting offspring are called clones because they’re genetically identical to the parent.
  1. We’ve seen the tissue culture as having an important impact in the field of agriculture, we’ve seen several important advantages of production with tissue-culture these include
  2. Improved propagation efficiency.
  3. the development of pathogen-free planting stock.
  4. the capability of storing germplasm for later use.
  5. Propagation by tissue culture also produces plants that are the exact replicate of the mother stock.
  6. Tissue culture labs vary in size and sophistication however all labs share several basic features whether large small it’s most important that aseptic working conditions are provided.
  7. That means the laboratory must be free of bacteria, fungi, and other microorganisms.
  8. All working surfaces tools and equipment should be regularly sterilized and kept free of contamination that may hinder plant growth.
  9. Typically, a tissue culture lab consists of three workspaces
  10. Preparation area.
  11. Transfer room
  12. Growth room
Let’s take a brief look at the four stages of the tissue culture process
Initiation: In the initiation, stage ex-plants are taken from the mother stock and established on a sterile nutrient media
Proliferation: In the proliferation stage the plantlets multiply and are divided and placed in a larger container
Pre-transplant: In the pre-transplant stage the plants are hardened off for conditions outside the laboratory.
Establishment: In the establishment stage the plants are transplanted and established in a greenhouse or nursery environment.
The tissue culture offers many advantages over traditional plant are as followed:
  1. Propagation space
  2. Labor and maintenance.
  3. The needs are dramatically lessened and vast quantities of pathogen-free plants can be produced in a relatively short period of time.
  4. With limited mother stock, the procedure isn’t affected by the changing seasons because work is done on the cellular level and not with the whole organism.
  5. Tissue culture offers an a time-saving alternative to conventional crossbreeding programs and makes combinations of totally unrelated plants possible.
  6. It’s also an efficient means by which genetic material can be stored for future study.
  7. But there are stumbling blocks to preventing the more widespread use of tissue culture.

Drawbacks of the tissue culture are as followed:

  1. It’s evident that startup costs for a facility and equipment can be substantial also skilled labor is needed to ensure profitable results.
  2. Plants produced by tissue culture are an issue such as they are quite small and must be given time to acclimate to conditions outside the lab.
  3. Any mistake at any stage of the procedure can cause contamination or genetic aberrations, ruining the crop
If you were to pull commercial tissue culture labs worldwide you’d discover that this agricultural pioneersanticipate a promising future.Tissue culture offers an efficient and economical solution to some of the major challenges facing all areas of agriculture in the 21st century.

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